LOCUS       Exported                3851 bp ds-DNA    circular SYN 17-OCT-2014
DEFINITION  .
ACCESSION   .
VERSION     .
KEYWORDS    piG0109_pluxRRR12y-sfGFP_EcoRI-_XbaI-
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 3851)
  AUTHORS   Daniel Gerngross
  TITLE     Direct Submission
  JOURNAL   Exported 17 Oct 2014 from SnapGene 2.5.0
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..3851
                     /organism="recombinant plasmid"
                     /mol_type="other DNA"
     terminator      1..126
                     /note="T0"
     terminator      27..121
                     /note="lambda t0 terminator"
                     /note="transcription terminator from phage lambda"
     misc_feature    127..196
                     /note="R2oDNA spacer"
                     /note="R2oDNA 70bp spacer 13"
     terminator      203..230
                     /note="T7Te"
                     /note="phage T7 early transcription terminator"
     misc_feature    complement(238..312)
                     /note="taR12y"
     promoter        complement(313..367)
                     /note="lux rR (R0062)"
     misc_feature    368..437
                     /note="spacer (B0040)"
     promoter        438..492
                     /note="lux rR (R0062)"
     3'UTR           493..548
                     /note="crR12y"
     RBS             531..542
                     /note="RBS (B0034)"
     CDS             549..1274
                     /codon_start=1
                     /note="sfGFP"
                     /translation="MSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYKRP*"
     terminator      1277..1393
                     /note="T1"
     terminator      1286..1372
                     /gene="Escherichia coli rrnB"
                     /note="T1"
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     1400..1419
                     /note="AB179"
     primer_bind     1585..1604
                     /note="AB5"
     rep_origin      1738..2565
                     /direction=RIGHT
                     /note="p15A"
     rep_origin      1986..1988
                     /direction=RIGHT
                     /note="P15 ori from pACYC184"
     rep_origin      complement(1989..2533)
                     /direction=LEFT
                     /note="p15A ori"
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin."
     primer_bind     2637..2656
                     /note="AB180"
     oriT            2667..2775
                     /note="incP origin of transfer"
     misc_feature    2747
                     /note="C->T no real mutation, just for testing"
     CDS             complement(2931..3746)
                     /note="KamR"
ORIGIN
        1 aaatcgtaat tattggggac ccctggattc tcaccaataa aaaacgcccg gcggcaaccg
       61 agcgttctga acaaatccag atggagttct gaggtcatta ctggatctat caacaggagt
      121 ccaagatatt cgtcacagta gttcaacctc agatacagaa accgtagatt gggattcagg
      181 aagttaccct attgtttata aacgcagaaa ggcccacccg aaggtgagcc agtgtgatct
      241 agctagagat atatggtagt agtaagttaa ttttcattaa ccaccactac caatcacctc
      301 ctggatttag cttttattcg actataacaa accattttct tgcgtaaacc tgtacgatcc
      361 tacaggtagg ttctgttaag taactgaacc caatgtcgtt agtgacgctt acctcttaag
      421 aggtcactga cctaacaacc tgtaggatcg tacaggttta cgcaagaaaa tggtttgtta
      481 tagtcgaata aagaattaat tctaccattc acctcttgga tttagctatt aaagaggaga
      541 aaggtaccat gagcaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat
      601 tagatggtga tgttaatggg cacaaatttt ctgtccgtgg agagggtgaa ggtgatgcta
      661 caaacggaaa actcaccctt aaatttattt gcactactgg aaaactacct gttccgtggc
      721 caacacttgt cactactctg acctatggtg ttcaatgctt ttcccgttat ccggatcaca
      781 tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaacgcacta
      841 tatctttcaa agatgacggg acctacaaga cgcgtgctga agtcaagttt gaaggtgata
      901 cccttgttaa tcgtatcgag ttaaagggta ttgattttaa agaagatgga aacattcttg
      961 gacacaaact cgagtacaac tttaactcac acaatgtata catcacggca gacaaacaaa
     1021 agaatggaat caaagctaac ttcaaaattc gccacaacgt tgaagatggt tccgttcaac
     1081 tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca
     1141 accattacct gtcgacacaa tctgtccttt cgaaagatcc caacgaaaag cgtgaccaca
     1201 tggtccttct tgagtttgta actgctgctg ggattacaca tggcatggat gagctctaca
     1261 aaaggcctta ataaattaaa ggcatcaaat aaaacgaaag gctcagtcga aagactgggc
     1321 ctttcgtttt atctgttgtt tgtcggtgaa cgctctcctg agtaggacaa atccgccgcc
     1381 ctagacagct gggcgcgcca aatacgcccg gtagtgatct tatttcatta tggtgaaagt
     1441 tggaacctct tacgtgccga tcaacgtctc attttcgcca aaagttggcc cagggcttcc
     1501 cggtatcaac agggacacca ggatttattt attctgcgaa gtgatcttcc gtcacaggta
     1561 tttattcggc gcaaagtgcg tcgggtgatg ctgccaactt actgatttag tgtatgatgg
     1621 tgtttttgag gtgctccagt ggcttctgtt tctatcagct gtccctcctg ttcagctact
     1681 gacggggtgg tgcgtaacgg caaaagcacc gccggacatc agcgctagcg gagtgtatac
     1741 tggcttacta tgttggcact gatgagggtg tcagtgaagt gcttcatgtg gcaggagaaa
     1801 aaaggctgca ccggtgcgtc agcagaatat gtgatacagg atatattccg cttcctcgct
     1861 cactgactcg ctacgctcgg tcgttcgact gcggcgagcg gaaatggctt acgaacgggg
     1921 cggagatttc ctggaagatg ccaggaagat acttaacagg gaagtgagag ggccgcggca
     1981 aagccgtttt tccataggct ccgcccccct gacaagcatc acgaaatctg acgctcaaat
     2041 cagtggtggc gaaacccgac aggactataa agataccagg cgtttcccct ggcggctccc
     2101 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
     2161 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
     2221 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
     2281 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
     2341 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
     2401 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
     2461 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
     2521 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctagc atgagcggat
     2581 acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa
     2641 aagtgccacc tgctagggcc ggcctacggc cagcctcgca gagcaggatt cccgttgagc
     2701 accgccaggt gcgaataagg gacagtgaag aaggaacacc cgctcgtggg tgggcctact
     2761 tcacctatcc tgcccggctg acgccgttgg atacaccaag gaaagtctac acgaaccctt
     2821 tggcaaaatc ctgtatatcg tgcgaaaaag gatggatata ccgaaaaaat cgctataatg
     2881 accccgaagc agggttatgc agcggaaaag gacccttgtc caattaatta ttagaaaaat
     2941 tcatccagca tcagatgaaa ttgcagtttg ttcatatccg gattatcaat gccatatttc
     3001 tgaaacagac gtttttgcag gctcgggcta aattcgccca ggcagttcca cagaatggcc
     3061 agatcctgat aacgatccgc aatgcccaca cggcccacat caatgcagcc aatcagtttg
     3121 ccttcatcga aaatcaggtt atccaggcta aaatcgccgt gggtcaccac gctatccggg
     3181 ctaaacggca gcagtttatg catttctttc cacacctgtt ccaccggcca gccgttacgt
     3241 tcatcatcaa aatcgctcgc atccaccagg ccgttgttca tacggctctg cgcctgggcc
     3301 agacgaaaca cacgatcgct gttaaacggg cagttgcaca ccggaatgct atgcagacga
     3361 cgcagaaaca cggccagcgc atccacaatg ttttcgccgc tatccggata ttcttccagc
     3421 acctgaaacg cggttttgcc cggaatcgcg gtggtcagca gccacgcatc atccggggtg
     3481 cgaataaaat gtttaatggt cggcagcggc ataaattcgg tcagccagtt cagacgcacc
     3541 atttcatcgg tcacatcgtt cgccacgctg cctttgccat gtttcagaaa cagttccggc
     3601 gcatccggtt tgccatacag acgataaatg gtcgcgccgc tctgacccac gttatcacgc
     3661 gcccatttat agccatacag atccgcatcc atgttgctgt tcagacgcgg acggctacag
     3721 ctcgtttcac gctgaatatg gctcataaca ccccttgtat tactgtttat gtaagcagac
     3781 agttttattg ttcatgatga tatattttta tcttgtgcaa tgtaacatca gagattttga
     3841 gacacaaatt t
//
